Genetic Diversity Analysis of Tomato (Solanum lycopersicum L.) with Morphological, Cytological, and Molecular Markers under Heat Stress
نویسندگان
چکیده
Tomatoes are usually consumed daily in the human diet. High temperatures reduce number of tomato yields per year. Heat stress has been considered one most prominent causes alterations morphological and molecular characteristics crops that decrease normal growth, production, yield diverse plants, including tomatoes (Solanum lycopersicum L.). In this study, we evaluated six lines, namely G1, G2, G3, G4, G5, G6, at morphological, molecular, cytological levels under heat stress. The average results two seasons (2018 2019) clarified G2 lines recorded highest flowering values, as well some fruit vegetative growth traits. Furthermore, G6 had maximum fruits/plant, whereas G1 produced yield/plant high temperatures. chromosomes all was 2n = 24, except for which 26, chromosome sizes were small, ranging from 323.08 to 464.48 µm. cultivar a symmetrical (primitive), having total form percentage (TF%) symmetry index (Syi) values minimum karyotype asymmetry (ASK) value, G4 asymmetrical (advanced). Molecular marker analysis demonstrated intersimple sequence repeat (ISSR) primers 49A, HB-14, 49B, 89B presented polymorphism P%, (MI), effective multiplex ratio (EMR), information content (PIC), respectively. contrast, OP-A3, OP-B3, SCoT 2, 12 showed PIC, EMR, MI, resolving power (Rp) across studied random amplified polymorphic DNA (RAPD) start codon-targeted (SCoT) primers. Moreover, ISSR revealed unique specific markers (6), followed by RAPD (4) (3) markers. Cluster combined data relating attributes separated G3 into group, other clustered another group. On whole, application using cytological, genetics techniques could be provide suitable parameters studying evolution genetic divergence between lines.
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ژورنال
عنوان ژورنال: Horticulturae
سال: 2021
ISSN: ['2311-7524']
DOI: https://doi.org/10.3390/horticulturae7040065